The technological advancements in the field of aquaculture have been achieved through enhanced husbandry protocols, improved genetic traits, nutritional diets, disease diagnosis and their control. With increasing demands for high quality aquaculture foods, more efficient and secure production system is needed. Therefore in the present investigation various experiments were performed to know the present status of Penaeus vannamei culture with special emphasis on the water quality parameters, growth and survival and immunological indices from the culture ponds of Ampalam and Gollalavalasa in Srikakulam district of Andhra Pradesh, India. A major constraint in aquaculture industry all over the world is loss due to diseases. According to FAO estimates, the loss is around $ 3,000 million per year. In India the loss is estimated to be about Rs. 600 crores per annum. Culture of shrimps in all geographical regions of the world has been routinely affected by various diseases, causing major loss due to pathogenic micro-organisms. To overcome this issue majority of the culture operators still using antibiotics and other chemical substances. Regular use of these may lead to existence of antibiotic resistance bacteria. To avoid these problems, in this study, the applications of different commercial probiotics of feed, soil and water were applied in the culture ponds (Super PS, Super biotic, Pond Plus, Pro-Tectand Pro-2) along with the immunostimulant 1,3 ?-Glucan, a commercial brand ?-ADVANTAGE. Interestingly promising results were achieved with 1,3 ?-Glucan in terms of hemocytic counts, total phagocytic and phenol oxidase activity.